#3687 KIDNEY TRANSCRIPTOME VARIES BETWEEN DONOR TYPES, WITH A DIFFERENTIAL REPONSE TO ISCHEMIC PRECONDITIONING

Abstract Background and Aims The prevention/attenuation of graft ischemic injury is a challenge in kidney transplantation. We developed two rat models to investigate the impact mesenchymal stromal cells (MSCs) preconditioning kidneys from Donors after Circulatory Death (DCD) Brain (DBD). Method Under general anesthesia, rats underwent iv injection saline (S-groups) or 1.5 106 MSCs (MSC-groups) followed by either DBD (6hr brain death) DCD anesthesia 20min warm ischemia) models, resulting 4 groups (S-DBD, S-DCD, MSC-DBD, MSC-DCD). Kidneys were then procured IGL1 flush. One was directly fixed other one immersed for 14 hours at 4°C. Serum samples collected before treatment (baseline) time collection. Urine bladder puncture Renal function evaluated. Kidney histology assessed PAS staining KIM1 immunostaining. Total RNA extracted S-DCD vs S-DBD RNAseq. Results BUN increased 6h death (DBD) (p<0.01). SCr both MSC-DBD but lower MSC-treated (MSC-DBD 0.5±0.2mg/dL 0.7±0.1mg/dL; p = 0.037). Urinary (S-DBD 10.9±4.5 7.1±1.7; 0.03). Acute Tubular Injury (ATI) expression higher (ATI: 65±24% surface 39±27% (p 0.03) KIM1: 0.39±0.24% 0.10±0.09% 0.0002)). In MSC groups, there no difference ATI extension expression. RNAseq showed that proinflammatory proapoptotic pathways upregulated DBD, whereas transmembrane transport metabolic downregulated, compared DCD. Conclusion profiles are different upon donor types, which may response MSC-based preconditioning.